Type
Recombinant
Description
CDNFisasecretedneurotrophicfactorthatisexpressedinbrain,neuronalandcertainnon-neuronaltissues.Ithasbeenshowntopromotesurvival,growthandfunctionofdopaminespecificneurons.CDNFanditsstructuralhomologMANF,eachcontainanN-terminalsaposin-likelipidbindingdomain,andacarboxyl-terminaldomain,whichisnothomologoustopreviouslycharacterizedproteinstructures.CDNFandMANFcanprevent6-OHDAinduceddegenerationofdopaminergicneuronsbytriggeringsurvivalpathwaysinaratexperimentalmodelofParkinsondisease.
AminoAcidSequence
MQEAGGRPGADCEVCKEFLNRFYKSLIDRGVNFSLDTIEKELISFCLDTKGKENRLCYYLGATKDAATKILSEVTRPMSVHMPAMKICEKLKKLDSQICELKYEKTLDLASVDLRKMRVAELKQILHSWGEECRACAEKTDYVNLIQELAPKYAATHPKTEL
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytostimulatetheproliferationofratC6cells.TheexpectedED50forthiseffectis15–25 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
