Type

Recombinant

Description

Total 76 AA. MW: 9,0 kDa (calculated). UniProtKB acc.no# P80098 (24–99) Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKL

Source

E. coli

Purity

> 97% by SDS PAGE

Biological Activity

EC50 = 0.44nM determined by Migration Assay with cells expressing recombinant CCR2

Endotoxin

<0.01 EU per 1µg of the protein

Reconstitution

Add sterile water to prepare a working stock solution of approximately 0.1 mg/ml and let the lyophilized pellet dissolve completely.

Applications

Western blotting, Cell culture and/or animal studies, Biologically active protein

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –20 to –70 °C. Lyophilized protein remains stable until the expiry date when stored at-20 to –70 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -20 to –70 °C, the protein is stable for 3 months at –20 to –70 °C under sterile conditions after reconstitution. Reconstituted protein can be stored at 4 °C for 1 month under sterile conditions after reconstitution

Quality Control Test

Quantification is done by A280, using the calculated extinction coefficient (8730 M-1 cm-1).
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.

Note

This product is intended for research use only.