Description

Total95 AA.MW:11,2 kDa(calculated).BiotinylatedatthelastLysineinsequence.UniProtKBacc.no#P80098(24–99)ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKLKLGSGLNDIFEAQKIEWHE

Source

E.coli

Purity

>97%bySDSPAGE

BIOLOGicalActivity

EC50=1.4–2.6 nMdeterminedbymigrationassaywithcellsexpressingrecombinant CCR2

Endotoxin

<0.01 EUper1µgoftheprotein

Reconstitution

Addsterilewatertoprepareaworkingstocksolutionofapproximately0.1 mg/mlandletthelyophilizedpelletdissolvecompletely.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –20to –70°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat-20 to –70°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat -20 to –70°C,theproteinisstablefor3 monthsat –20to –70°Cundersterileconditionsafterreconstitution.Reconstitutedproteincanbestoredat4 °Cfor1 monthundersterileconditionsafterreconstitution

Note

Thisproductisintendedforresearchuseonly.