Type

Recombinant

Description

Total88 AA.MW:10,1 kDa(calculated).UniProtKBacc.noQ9Y4X3(25–112)ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSLSQWFEHQERKLHGTLPKLNFGMLRKMG

Source

E.coli

Purity

PurityasdeterminedbySDSPAGE:>97%

BIOLOGicalActivity

EC50=34nMdeterminedbyMigrationAssayincellsexpressingrecombinant CCR10

Endotoxin

<0.01 EU/1µg

Reconstitution

Addsterilewatertoprepareaworkingstocksolutionofapproximately0.1 mg/mlandletthelyophilizedpelletdissolvecompletely.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies,Biologicallyactiveprotein

Shipping

Addsterilewatertoprepareaworkingstocksolutionofapproximately0.1 mg/mlandletthelyophilizedpelletdissolvecompletely.

Storage/Expiration

Storethelyophilizedproteinat –20to –70°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat-20 to –70°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat -20 to –70°C,theproteinisstablefor3 monthsat –20to –70°Cundersterileconditionsafterreconstitution.Reconstitutedproteincanbestoredat4 °Cfor1 monthundersterileconditionsafterreconstitution

QualityControlTest

QuantificationisdonebyA280,usingthecalculatedextinctioncoefficient(7450 M-1 cm-1).
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.

Note

Thisproductisintendedforresearchuseonly.