Type

Recombinant

Description

CT-1 isamemberoftheIL-6 familyofcytokineswhichalsoincludesLIF,CNTF,OSM(OncostatinM),IL-11,IL-6 andpossIBLyNNT-1/BSF-3. CT-1 isapleiotropiccytokinewhichisexpressedinvarioustissuesincludingtheadultheart,skeletalmuscle,ovary,colon,prostateandfetallung,andsignalsthroughtheLIFreceptorandthegp130receptorsubunit.CT-1 hastheABIlitytoinducecardiacmyocytehypertrophy,andenhancesthesurvivalofcardiomyocyteanddifferentneuronalpopulations.RecombinantMurineCardiotrophin-1 isa21.3 kDaproteinconsistingof202 aminoacidresidues.

AminoAcidSequence

SQREGSLEDHQTDSSISFLPHLEAKIRQTHNLARLLTKYAEQLLEEYVQQQGEPFGLPGFSPPRLPLAGLSGPAPSHAGLPVSERLRQDAAALSVLPALLDAVRRRQAELNPRAPRLLRSLEDAARQVRALGAAVETVLAALGAAARGPGPEPVTVATLFTANSTAGIFSAKVLGFHVCGLYGEWVSRTEGDLGQLVPGGVA

Source

E.coli

Purity

98%

BIOLOGicalActivity

TheED₅₀asdeterminedbythedose-dependentproliferationofTF-1 cellswas1.0 ng/ml,correspondingtoaspecificactivityof1×10⁶units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C