Type

Recombinant

Description

MurineC10belongstotheCCchemokinefamilyandisexpressedinmyelopoieticbonemarrowcultureswhenstimulatedwithGM-CSF,M-CSF,IL-3 orIL-4. ItsignalsprimarilythroughtheCCR1receptor.C10ischemotacticforBcells,CD4+Tcells,monocytesandNKcellsandalsoexhibitspowerfulsuppressiveactivityoncolonyformationbydifferentlineagesofhematopoieticProgenitors.TheC10containsthefourhighlyconservedcysteineresiduespresentinCCchemokines.Thematureproteincontains95 aminoacidresidues.RecombinantMurineC-10 isa10.7 kDaproteincontaining95 aminoacidresidues.

AminoAcidSequence

GLIQEIEKEDRRYNPPIIHQGFQDTSSDCCFSYATQIPCKRFIYYFPTSGGCIKPGIIFISRRGTQVCADPSDRRVQRCLSTLKQGPRSGNKVIA

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattractBalb/cmousespleenMNCsusingaconcentrationrangeof10.0–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C