Description

Brain-DerivedNeurotrophicFactorHumanRecombinantproducedinE.coliisahomodimer,non-glycosylated,polypeptidechaincontaining2×119aminoacidsandhavingatotalmolecularmassof26,984 Dalton.BDNFHumanRecombinantispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-His-Ser-Asp-Pro.

Source

E.coli

Purity

Greaterthan96.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheED50,calculatedbythedose-dependantinductionofACHE(acetylcholineesterase)inratbasalforebrainprimaryseptalcultureis50 ng/ml.

Formulation

Theproteinwaslyophilizedafterdialysisagainst20 mMsodiumcitratebuffer pH=5.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedBDNFalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionIFN-gammashouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.