Description

Brain-Derived Neurotrophic Factor Human Recombinant produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2×119 amino acids and having a total molecular mass of 26,984 Dalton. BDNF Human Recombinant is purified by proprietary chromatographic techniques.

Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ser-Asp-Pro.

Source

E. coli

Purity

Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Biological Activity

The ED50, calculated by the dose-dependant induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml.

Formulation

The protein was lyophilized after dialysis against 20 mM sodium citrate buffer pH=5.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.