Type
Recombinant
Description
Bonemorphogeneticproteins(BMPs)constituteasubfamilywithintheTGF-βsuperfamilyofstructurallyrelatedsignalingproteins.Membersofthissuperfamilyarewidelydistributedthroughoutthebody,andareinvolvedindiversephysiologicalprocessesduringbothpre-andpostnatallife.LikeBMP-7,BMP-4 isinvolvedinthedevelopmentandmaintenanceofboneandcartilage.ReducedexpressionofBMP-4 isassociatedwithanumberofbonediseases,includingtheheritabledisorderFibrodysplasiaOssificansProgressiva.PeproTech’s E.coliderivedmurineBMP-4 isafullyactivehomodimericproteinconsistingoftwo106 aminoacidsubunitswhichcorrespondtoaminoacids303–408ofthefulllengthBMP-4 precursor.ThecalculatedmolecularweightofRecombinantMurineBMP-4 is23.9 kDa.
AminoAcidSequence
KKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5 cells.TheexpectedED50forthiseffectis5–15 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein10mMCitricAcidtoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
