Type
Recombinant
Description
BoneMorphogeneticProtein-2 HumanRecombinantproducedinE.Coliisahomodimeric,non-glycosylated,Polypeptidechaincontaining115 aminoacidsandhavingamolecularmassof26018 Dalton.TheBMP-2 ispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Gln-Ala-Lys-His.
Source
E.coli
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
BIOLOGicalActivity
1. TheED50asdeterminedbythecytolysisofMC3T3-E1cellsis<50 ng/ml.2. Whenimplantedinthemousethighbonemuscleandgrowfor14 days,Muscletissuewasharvestedandexaminedbiochemicallyforcalciumuptake.Ourunitdefinedas1 microgramcalciumabsorbed.Thespecificactivityismorethan1000 Units/mg.3. TheactivityofBMP-2 wastestedusingalkalinephosphatase,theactivityinmousemusclemyoblastC2C12celllinewasfoundtobe1×105U/mgprotein.4. Theectopicosteogenesiscapacityis1000mgfreshboneper1mgof BMP-2.
Formulation
BMP-2 waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining20mMNaAc pH=4.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
LyophilizedBoneMorphogeneticProtein-2 althoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionBMP2shouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.
