Type
Recombinant
Description
TGF-βfamilymembersarekeymodulatorsofcellproliferation,differentiation,matrixsynthesis,andapoptosis.Asimpliedbytheirname,BMPsinitiate,promote,andregulatethedevelopment,growth,andremodelingofboneandcartilage.Inadditiontothisrole,BMPsarealsoinvolvedinprenataldevelopmentandpostnatalgrowth,remodeling,andmaintenanceofavarietyofothertissuesandorgans.BMP-5 isexpressedinthenervoussystem,lungsandliver.Itisaknownregulatorfordendriticgrowthinsympatheticneurons.BMP-5 isa454 aminoacidprecursorproteinthatiscleavedtoreleasetheBIOLOGicallyactiveC-terminalmatureprotein.RecombinantHumanBMP-5 isa31.2 kDahomodimericdisulfide-linkedproteinconsistingoftwoidentical138 aminoacidchains.
AminoAcidSequence
AANKRKNQNRNKSSSHQDSSRMSSVGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH
Source
ChineseHamsterOvaryCells(CHO)
Purity
95%
BiologicalActivity
DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5 cells.TheexpectedED50forthiseffectis0.5–1.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
