Type

Recombinant

Description

Total193 AA.MW:22.0 kDa(calculated).Acc.no.NP_001258639(Val16-Ala198).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASVRPAPVAPLGGPEPAQYEELTLLFHGALQLGQALNGVYKATEARLTEAGRNLGLFDQALEFLGREVNQGRDATRELRTSLSEIQAEEDTLHLRAEATARSLREVARAQHALRNSVRRLQVQLRGAWLGQAHQEFENLKDRADKQNHLLWALTGHVQRQQREMAEQQQWLRQIQQRLHMAALPA

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:> 95%

SDS-PAGEGel

14 %SDS-PAGEseparationofRatBetatrophin:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,4 μg/lane
3.Non-reducedandnon-boiledsample,4 μg/lane

Endotoxin

<1.0 EU/μg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.03MAcetatebufferpH 4.0

Reconstitution

Add0.1 MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10 μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.