Type
Recombinant
Description
β-NGFisaneurotrophicfactorstructurallyrelatedtoBDNF,NT-3 andNT-4. Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.β-NGFisapotentneurotrophicfactorthatsignalsthroughitsreceptorβ-NGFR,andplaysacrucialroleinthedevelopmentandpreservationofthesensoryandsympatheticnervoussystems.β-NGFalsoactsasagrowthanddifferentiationfactorforBlymphocytes,andenhancesB-cellsurvival.ThefunctionalformofRecombinantMurineβ-NGFisanon-covalently-linkedhomodimeroftwo13.4 kDa,polypeptidemonomersthateachcontain120 aminoacidsandthreedisulfidebonds,whicharerequiredforBIOLOGicalactivity.
AminoAcidSequence
SSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofhumanTF-1 cellsis≤1.0 ng/ml,correspondingtoaspecificactivityof≥1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
