Type
Recombinant
Description
BetacellulinisanEGF-relatedpolypeptidegrowthfactorthatsignalsthroughtheEGFreceptor.Itisproducedinseveraltissues,includingthepancreas,smallintestine,andincertaintumorcells.Betacellulinisapotentmitogenforretinalpigmentepithelialcellsandvascularsmoothmusclecells.Betacellulinisinitiallysynthesizedasaglycosylated32.0 kDatransmembraneprecursorprotein,whichisprocessedbyproteolyticcleavagetoproducethematuresequence.RecombinantMurineBetacellulinisa9.0 kDamonomericprotein,containing80 aminoacidresidues,whichcomprisesthematureEGF-homologousportionoftheBetacellulinprotein.
AminoAcidSequence
DGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCPKQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFY
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytostimulatetheproliferationofmouseBalb/3T3cells. TheexpectedED50is0.01≤ng/ml,correspondingtoaspecificactivityof≥1×108units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
