Type
Recombinant
Description
BetacellulinisanEGF-relatedpolypeptidegrowthfactorthatsignalsthroughtheEGFreceptor.Itisproducedinseveraltissues,includingthepancreas,smallintestine,andincertaintumorcells.Betacellulinisapotentmitogenforretinalpigmentepithelialcellsandvascularsmoothmusclecells.HumanBetacellulinisinitiallysynthesizedasaglycosylated32.0 kDatransmembraneprecursorprotein,whichisprocessedbyproteolyticcleavagetoproducethematuresequence.RecombinantHumanBetacellulinisa9.0 kDamonomericprotein,containing80 aminoacidresidues,whichcomprisesthematureEGF-homologousportionoftheBetacellulinprotein.
AminoAcidSequence
DGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCPKQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFY
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineBalb/3T3cellsis≤0.05 ng/ml,correspondingtoaspecificactivityof≥2×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
