Type

Recombinant

Description

Defensins(alphaandbeta)arecationicpeptideswithabroadspectrumofantimicrobialactivitythatcompriseanimportantarmoftheinnateimmunesystem.Theα-defensinsaredistinguishedfromtheβ-defensinsbythepairingoftheirthreedisulfidebonds.Todate,sixhumanβ-defensinshavebeenidentified;BD-1,BD-2,BD-3,BD-4,BD-5 andBD-6. β-defensinsareexpressedonsomeleukocytesandatepithelialsurfaces.Inadditiontotheirdirectantimicrobialactivities,theycanactaschemoattractantstowardsimmaturedendriticcellsandmemoryTcells.Theβ-defensinproteinsareexpressedastheC-terminalportionofprecursors,andarereleasedbyproteolyticcleavageofasignalsequenceand,insomecases,apropeptidesequence.β-defensinscontainasix-cysteinemotifthatformsthreeintra-moleculardisulfidebonds.BD-4 isexpressedinthetestes,stomach,uterus,neutrophils,thyroid,lungsandkidneys.Inadditiontoitsdirectantimicrobialactivities,BD-4 ischemoattractanttowardshumanbloodmonocytes.RecombinantHumanBD-4 isa6.0 kDaproteincontaining50 aminoacidresidues.

AminoAcidSequence

EFELDRICGYGTARCRKKCRSQEYRIGRCPNTYACCLRKWDESLLNRTKP

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesusingaconcentrationrangeof0.1–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstitutein10mMAceticAcidtoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C