Type

Recombinant

Description

BCA-1/BLC,aCXCchemokine,isexpressedintheliver,spleen,lymphnodes,appendixandstomach.Itexertsitsactivitiesthroughitsonlyreceptor,CXCR5.BCA-1/BLCisapotentchemoattractantforBlymphocytes,andinducesweakchemotacticresponseinTcellsandmacrophages.Itmanifestsnoactivityonneutrophilsandmonocytes.RecombinantHumanBCA-1 isa10.3 kDaproteincontaining87 aminoacidresiduesincludingthefourhighlyconservedcysteineresiduespresentinCXCchemokines.

AminoAcidSequence

VLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanBcellsusingaconcentrationrangeof1.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C