Type

Recombinant

Description

Arteminisadisulfide-linkedhomodimericneurotrophicfactorstructurallyrelatedtoGDNF,Artemin,NeurturinandPersephin.Theseproteinsbelongtothecysteineknotsuperfamilyofgrowthfactorsthatassumestabledimericproteinstructures.Artemin,GDNF,PersephinandNeurturinallsignalthroughamulticomponentreceptorsystem,composedofRET(receptortyrosinekinase)andoneofthefourGFRα(α1-α4)receptors.ArteminprefersthereceptorGFRα3-RET,butwilluseotherreceptorsasanalternative.Arteminsupportsthesurvivalofallperipheralganglia,suchassympathetic,neuralcrestandplacodally-derivedsensoryneurons,anddopaminergicmidbrainneurons.ThefunctionalhumanArteminligandisadisulfide-linkedhomodimeroftwo12.0 kDapolypeptidemonomers.Eachmonomercontainssevenconservedcysteineresidues,oneofwhichisusedforinterchaindisulfidebridgingandtheothersareinvolvedinintramolecularringformationknownasthecysteineknotconfiguration.RecombinantHumanArteminisa24.2 kDa,disulfide-linkedhomodimerformedbytwoidentical113 aminoacidsubunits.

AminoAcidSequence

AGGPGSRARAAGARGCRLRSQLVPVRALGLGHRSDELVRFRFCSGSCRRARSPHDLSLASLLGAGALRPPPGSRPVSQPCCRPTRYEAVSFMDVNSTWRTVDRLSATACGCLG

Source

E.coli

Purity

98%

BIOLOGicalActivity

Determinedbyit"s ABIlitytopromotesurvivalandneuriteoutgrowthanddorsalrootganglionneurons.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–0.5 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C