Type

Recombinant

Description

Total175 AA.MW:20.1 kDa(calculated).UniProtKBacc.No.P05090(Gln21-Ser189).C-terminalHis-tag(6 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

QAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLSHHHHHH

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis:>80 %

SDS-PAGEGel

12%SDS-PAGEseparationofHumanApoD(HEK)
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/lane

Endotoxin

<0.1 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.