Type

Recombinant

Description

Total 174 AA, MW: 19.82 kDa (calculated). C-terminal His-tag, 7 extra AA (highlighted). E280 = 26150 M-1cm-1. The amino acid sequence of this product corresponds to the UniProtKB/Swiss-Prot entry P05090 (Human ApoD). Following modifications were made: Four amino acid exchanges were introduced at the surface of ApoD (Trp99His, Cys116Ser, Ile118Ser, Leu120Ser) to enhance the solubility of the recombinant protein and another three (Leu23Pro, Pro133Val, Asn134Ala) to facilitate its genetic manipulation (highlighted).

Amino Acid Sequence

FHLGKCPNPPVQENFDVNKYPGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYHILATDYENYALVYSCTSISQSFHVDFAWILARNVALPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLSAHHHHHH

Source

E. coli

Purity

>95%

SDS-PAGE Gel

12% SDS-PAGE separation of Human ApoD
1. MW marker – 97, 66, 45, 31, 21, 14 kDa
2. non reduced and non boiled sample, 5 μg / lane
3. reduced and boiled sample, 5 μg / lane

Endotoxin

<1.0 EU/µg

Formulation

Filtered (0.4 μm) and lyophilized from 1.0 mg/mL in 4 mM KH2PO4; 16 mM Na2HPO4; 115 mM NaCl; pH 7.5

Reconstitution

Add deionized water to a working concentration approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.