Type

Recombinant

Description

ApoA-I isa29.0 kDaproteinproducedintheliverandintestine,andsecretedasthepredominantconstituentofnascenthighdensitylipoprotein(HDL)particle.ApoA-I,whichisfoundexclusivelyinHDL,hasauniqueABIlitytocaptureandsolubilizefreecholesterol.ThisApoA-I abilityenablesHDLtoremoveexcessperipheralcholesterol,andreturnittotheliverforrecyclingandexcretion.Thisprocess,calledreversecholesteroltransport,isthoughttoinhibitatherogenesis.Forthisreason,HDLisalsoknownasthe“goodcholesterol.”ThetherapeuticpotentialofApoA-I hasbeenrecentlyassessedinpatientswithacutecoronarysyndromes,usingarecombinantformofanaturallyoccurringvariantofApoA-I(calledApoA-I Milano).TheavailabilityofrecombinantnormalApoA-I shouldfacilitatefurtherinvestigationintothepotentialusefulnessofApoA-I inpreventingatheroscleroticvasculardiseases.RecombinantHumanApoA-I isa28.2 kDaproteinof244 aminoacidresidues.

AminoAcidSequence

MDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARL

Source

E.coli

Purity

97%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C