Type
Native
Description
NativeproteinisolatedfromHumanAdiposeTissue,338 AA,MW38,472 kDa(calculatedwithoutglycosylation).ProteinidentityconfirmedbyMALDI-TOF/TOF(NCBIno.gi|4757756).
AminoAcidSequence
STVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Source
Humanadiposetissue
Purity
Purityasdeterminedbydensitometricimageanalysis:>90 %
SDS-PAGEGel
SDS-PAGEanalysisofAnnexinA2NATIVE,HumanAdiposeTissue12%gelstainedwithCoomassieBrillantBlueR250
- M.W.Marker –14,21,31,45,66,97 kDa
- reducedandboiledsample,2.5μg/lane
- nonreducedandnonboiledsample,2.5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm)andlyophilizedin0,5 mg/mLin0,05Mphosphatebuffer,0,075MNaCl,pH 6,5.
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies,Immunologicalmethods
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
