Type

Recombinant

Description

Total462 AA.MW:51.0 kDa(calculated).UniProtKBacc.No.P01019(Asp34-Ala485).C-terminallinker(2 extraAA)andC-terminalFLAG-tag(8 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

DRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLTGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVSFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTARTDYKDDDDK

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanAGT:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5 μg/lane
3.non-reducedandnon-heatedsample,2.5 μg/lane

Endotoxin

<0.1 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLsolutionin20 mMTrisbuffer,50 mMNaCl,pH 7.5.

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cforthree days.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.