Type

Recombinant

Description

Total481 AA.MW:56 kDa(calculated).UniProtKBacc.no.Q9UKU9(Ser33-Ile193).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASQEDGFEGTEEGSPREFIYLNRYKRAGESQDKCTYTFIVPQQRVTGAICVNSKEPEVLLENRVHKQELELLNNELLKQKRQIETLQQLVEVDGGIVSEVKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNALELSQLENRILNQTADMLQLASKYKDLEHKYQHLATLAHNQSEIIAQLEEHCQRVPSARPVPQPPPAAPPRVYQPPTYNRIINQISTNEIQSDQNLKVLPPPLPTMPTLTSLPSSTDKPSGPWRDCLQALEDGHDTSSIYLVKPENTNRLMQVWCDQRHDPGGWTVIQRRLDGSVNFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTMEDWSGRKVFAEYASFRLEPESEYYKLRLGRYHGNAGDSFTWHNGKQFTTLDRDHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGVYWAEFRGGSYSLKKVVMMIRPNPNTFH

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:>95 %

SDS-PAGEGel

14%SDS-PAGEseparationofHumanAngiopoietin-LikeProtein2:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.03MAcetatebufferpH 4.0

Reconstitution

Add0.1MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.