Type

Recombinant

Description

AmphiregulinisanEGFrelatedgrowthfactorthatsignalsthroughtheEGF/TGF-αreceptor,andstimulatesgrowthofkeratinocytes,epithelialcellsandsomefibroblasts.Amphiregulinalsoinhibitsthegrowthofcertaincarcinomacelllines.Synthesizedasatransmembraneprotein,Amphiregulin’s ex­tracellulardomainisproteolyticallyprocessedtoreleasethematureprotein.Thereare6 conservedcysteineresidues,whichform3 intramoleculardisulfidebondsessentialforBIOLOGicalactivity.RecombinantHumanAmphiregulinisan11.3 kDaglycoproteinconsistingof98 aminoacidresidues.

AminoAcidSequence

SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSK

Source

E.coli

Purity

95%

BiologicalActivity

DeterminedbyitsABIlitytostimulatetheproliferationofmouseBalb/c3T3cells.TheexpectedED₅₀forthiseffectis5–10 ng/ml.

Storage/Expiration

 –20°C