Type
Native
Description
Nativeproteinisolatedfromhumanpooledserum,183 AA,MW21,560 kDa(calculatedwithoutglycosylation).
AminoAcidSequence
QIPLCANLVPVPITNATLDQITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES
Source
Humanpooledserum
Purity
Purityasdeterminedbydensitometricimageanalysis: >95%
SDS-PAGEGel
SDS-PAGEanalysisofHumanAlpha-1-acidglycoprotein1 NATIVEprotein12%gelstainedwithCoomassieBrillantBlueR250
- M.W.Marker –14,21,31,45,66,97 kDa
- reducedandheatedsample,2.5μg/lane
- non-reducedandnon-heatedsample,2.5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7,5
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies,Immunologicalmethods
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.AllbloodsamplesusedforproteinpreparationweretestedandfoundnegativeforHBsAg,anti-HCV,HIVAg/Ab,andsyphilis.Sincenotestcanabsolutelyassuretheabsenceofallinfectiousagents,thisproductshouldbehandledasapotentialbiohazard.
