Type

Recombinant

Description

Total155 aa;MW17,7 kDa(calculated);UniProtKBacc.no.P55008;N-terminalHis-tag;9 extraaminoacids(highlighted);

AminoAcidSequence

MKHHHHHHASQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

Source

E.coli

SDS-PAGEGel

14%SDS-PAGEseparationofHumanAIF-1
1. reducedandheatedsample,5μg/lane
2.M.W.Marker –14,21,31,45,66,97 kDa
3.non-reducedandnon-heatedsample,5μg/lane

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.