Type

Recombinant

Description

Total331 AA,MW:35.2 kDa(calculated).C-terminalFlag-tag11 AA(highlighted).

AminoAcidSequence

AQNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLAAAADYKDDDDK

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumansRAGE
1. MWMarker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5 μg/lane
3.nonreducedandnonheatedsample,2.5 μg/ lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLin20 mMTris,50 mMNaCl,pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.