Type

Recombinant

Description

Total238 AA.MW:25.7 kDa(calculated).UniProtKBacc.No.Q8K3R4(Gly18–Asn244).C-terminallinker(3 extraAA)andC-terminalFlag-tag(8 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

GITATEGPGALVPPPKETCAGWMAGIPGYPGHNGIPGRDGRDGTPGEKGEKGDAGVLGPKGDPGDAGMTGAEGPRGFPGTPGRKGEPGEAAYMYHSAFSVGLETRVTVPNVPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQYQEKNVDQASGSMLLHLEVGDQVWLQVYGEGDNNGLYADNVNDSTFTGFLLYHDTNAAADYKDDDDK

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

14 %SDS-PAGEseparationofRatAdiponectin:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin20 mMTrisbuffer,50 mMNaCl,pH 7.5.

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.