Type

Recombinant

Description

Total238 AA.MW:26.0 kDa(calculated).N-TerminalFlag-tag,UniProtKB/TrEMBLQ6PP07with13 extraAA(highlighted).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

HVDYKDDDDKPAGETTEKPGALLPMPKGACAGWMAGIPGHPGHNGTPGRDGRDGVPGEKGEKGDTGLTGPKGDTGESGVTGVEGPRGFPGIPGRKGEPGESAYVYRSAFSVGLETRVTVPNMPIRFTKIFYNQQNHYDVTTGKFHCNIPGLYYFSFHITVYLKDVKVSLYKKDKAVLFTYDQYQDKNVDQASGSVLLYLEKGDQVWLQAYGDEENNGVYADNVNDSIFTGFLLYHNIE

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

12%SDS-PAGEseparationofPorcineAdiponectin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.non-reducedandnon-heatedsample,2.5μg/lane
3.reducedandheatedsample,2.5μg/ lane

Endotoxin

<0.1 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.