Type
Recombinant
Description
Adiponectinisanadipose-derivedsecretedproteincontaining236 aminoacidresidues.Itisrelativelyabundantinhumansandrodents,accountingforabout0.01%oftotalplasmaprotein.Thecirculatinglevelsofadiponectinaredecreasedunderconditionsofobesity,insulinresistance,andtypeIIdiabetes.Disruptionofadiponectininmicecausesinsulinresistanceandneointimalformation.Conversely,admiNISTrationofrecombinantadiponectinsuppresseshepaticglucoseproduction,andreversesinsulinresistanceassociatedwithbothlipoatrophyandobesity.Theprotectiveroleofadiponectinisattributedtoitsanti-inflammatoryproperties(e.g.ABIlitytosuppressexpressionofTNF-αandclassA scavengerreceptorinmacrophages).RecombinantadiponectinisamultimericglycoproteincontainingaminoacidsVal-21 toAsn-247 oftheadiponectinprecursorproteinfusedtoanN-terminalhistidinetag.Monomericglycosylatedadiponectinmigratesatanapparentmolecularweightofapproximately35.0 kDabySDSPAGEanalysisunderreducingconditions.ThecalculatedmolecularweightofRecombinantMurineAdiponectinis25.8 kDa.
AminoAcidSequence
RGHHHHHHHHVTTTEELAPALVPPPKGTCAGWMAGIPGHPGHNGTPGRDGRDGTPGEKGEKGDAGLLGPKGETGDVGMTGAEGPRGFPGTPGRKGEPGEAAYVYRSAFSVGLETRVTVPNVPIRFTKIFYNQQNHYDGSTGKFYCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQYQEKNVDQASGSVLLHLEVGDQVWLQVYGDGDHNGLYADNVNDSTFTGFLLYHDTN
Source
Hi-5Insectcells
Purity
95%
BIOLOGicalActivity
DeterminedbyacytotoxicassayusingM1cells.TheED50forthiseffectis4.0–6.0 µg/ml.
Storage/Expiration
–20°C
