Type

Recombinant

AminoAcidSequence

EDDVTTTEELAPALVPPPKGTCAGWMAGIPGHPGHNGTPGRDGRDGTPGEKGEKGDAGLLGPKGETGDVGMTGAEGPRGFPGTPGRKGEPGEAAYMYRSAFSVGLETRVTVPNVPIRFTKIFYNQQNHYDGSTGKFYCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQYQEKNVDQASGSVLLHLEVGDQVWLQVYGDGDHNGLYADNVNDSTFTGFLLYHDTNDYKDDDDK

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >98%

SDS-PAGEGel

12%SDS-PAGEseparationofMouseA diponectin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,5μg/lane
3.non-reducedandnon-boiledsample,5μg/lane

BIOLOGicalActivity

Full-lengthadiponectinhasbeenshowntoactivateAMP-activatedproteinkinaseinhepatocyte.ItcanalsoactivateAMPKinHepG2humanhepatocytesattheconcentrationofaslowas1.0 µg/ml.Invitrogluconeogenesisassayinprimaryrathepatocyteswasperformed,showingthemurineadiponectinderivedfrommammaliancellscaninhibitglucoseproduction.

Endotoxin

<0.1 EU/ug

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Filteryourculturemedia/workingsolutionscontainingthisproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

GFCtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.