Type

Recombinant

Description

Total237 AA.MW:25.8 kDa(calculated).UniProtKBacc.No.Q15848(Glu19–Asn244).C-terminalFLAG-tag(11 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTNAAADYKDDDDK

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis:> 95%

SDS-PAGEGel

4–20%SDS-PAGEseparationofHumanAdiponectin:
1. M.W.Marker –14,21,31,45,66,97 kDa
2. Reducedandboiledsample,2.5 μg/lane
3. Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<0.1 EU/μg

Formulation

Sterilefiltered(0.2 μm)andfrozen0.4–1.0 mg/mlsolutioninphosphatebufferedsaline,pH 7.4.

Reconstitution

Diluteinsterilebuffersandmediatoaworkingconcentrationbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

Note

Thisproductisintendedforresearchuseonly.