Type

Recombinant

Description

Total226 AA,UniProtQ15848(Glu19 –Asn244).MW(monomer):24.6 kDa(calculated).Itmigratesat884 kDa(multimer).

AminoAcidSequence

ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN

Glu1 toGln5 wereconfirmedbyN-terminalsequencing

Source

HEK293

Purity

>90%

SDS-PAGEGel

4–20%gradientSDS-PAGEseparationAdiponectinHMW-richHumanHEK293,Tagfree
1. M.W.Marker –14,21,31,45,66,97 kDa
2. reducedandboiledsample,2.5μg/lane
3. non-reducedandnon-boiledsample,2.5μg/lane

BIOLOGicalActivity

EffectofthisproductonPDGF-inducedproliferationofhumanaorticsmoothmusclecellswasconfirmedexperimentaly.

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin20 mMTris,50 mMNaCl,pH7.5 and1mMCaCl2at0.5 mg/mLconcentration.

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

GFCtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.