Type

Recombinant

Description

Total 226 AA, UniProt Q15848 (Glu19 – Asn244). MW (monomer): 24.6 kDa (calculated). It migrates at 884 kDa (multimer).

Amino Acid Sequence

ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN

Glu 1 to Gln 5 were confirmed by N-terminal sequencing

Source

HEK293

Purity

>90%

SDS-PAGE Gel

4–20% gradient SDS-PAGE separation Adiponectin HMW-rich Human HEK293, Tag free
1. M.W. marker –14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5μg/lane
3. non-reduced and non-boiled sample, 2.5μg/lane

Biological Activity

Effect of this product on PDGF-induced proliferation of human aortic smooth muscle cells was confirmed experimentaly.

Endotoxin

<1.0 EU/ug

Formulation

Filtered (0.4 μm) and lyophilized in 20 mM Tris, 50 mM NaCl, pH 7.5 and 1mM CaCl2 at 0.5 mg/mL concentration.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA, Cell culture and/or animal studies

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

GFC to determine purity of the protein.

LAL to determine quantity of endotoxin.