Type

Recombinant

Description

Total171AA.N-terminalHis-tagandtheproteasecleavagesite,31 extraAA(highlighted).TheAAsequenceishomologoustotheaminoacidsequence(105–244)oftheHumanfull-lengthAdiponectin.

AminoAcidSequence

MSWWHHHHHHNWNIPTTQDTTQDLWFEGAMGGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN

Source

E.coli

Purity

>95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanglobularAdiponectin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Filteryourculturemedia/workingsolutionscontainingthisproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Invitro,Exvivoandinvivoactivityanalysis,Bindingassay

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

GFCtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.