Type
Recombinant
Description
ActivinBisaTGF-βfamilymemberthatexhibitsawiderangeofBIOLOGicalactivities,includingregulationofembryogenesis,osteogenesis,hematopoiesis,reproductivephysiologyandhormonesecretionfromthehypothalamic,pituitaryandgonadalglands.ActivinB,likecertainothermembersoftheTGF-βfamily,signalsthroughtheActRIIreceptor(ActivinReceptortypeII).Activinsarehomodimersorheterodimersofdifferentβsubunits.TheyareproducedasprecursorproteinswithanaminoterminalpropeptidethatiscleavedtoreleasetheC-terminalbioactiveligand.HumanActivinBisa25.6 kDadisulfide-linkedhomodimerconsistingoftwoβBchains,eachcontaining115 aminoacidresidues.
AminoAcidSequence
GLECDGRTNLCCRQQFFIDFRLIGWNDWIIAPTGYYGNYCEGSCPAYLAGVPGSASSFHTAVVNQYRMRGLNPGTVNSCCIPTKLSTMSMLYFDDEYNIVKRDVPNMIVEECGCA
Source
(BTI-Tn-5B1-4)Hi-5Insectcells*
Purity
95%
BiologicalActivity
TheED50asdeterminedbyitsABIlitytoinhibittheproliferationofmurineMPC-11 cellsis2.0 ng/ml,correspondingtoaspecificactivityof5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Storage/Expiration
–20°C