Type

Recombinant

Description

4–1BBL,amemberoftheTNFsuperfamily,isexpressedinBcells,dendriticcells,activatedTcellsandmacrophages. 4–1BBLbindstoitsreceptor4–1BBandprovidesaco-stimulatorysignalforTcellactivationandexpansion. Thehuman4–1BBLgenecodesfora254 aminoacidtypeIItransmembranecontaininga28 aminoacidcytoplasmicdomain,a21 aminoacidtransmembranedomainanda205 aminoacidextracellulardomain. Thesolubleformof4–1BBLcontainstheTNF-likeportionoftheextracellulardomainof4–1BBL. Recombinanthuman4–1BBLisasoluble19.5 kDaproteinconsistingof185 aminoacidresidues.

AminoAcidSequence

MREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE

Source

E.coli

Purity

98%

BIOLOGicalActivity

Determinedbythedose-dependentstimulationofIL-8 productionbyhumanPBMC. TheED50forthiseffectis5–10ng/ml.Note:ResultsmayvarywithPBMCdonors.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C