Type

Recombinant

Description

IL-12 isadisulfide-linkedheterodimericprotein(p70),composedoftwosubunits,p35andp40,whichareencodedbytwodifferentgenes.Accumulatingdataindicatesthatp40secretionprecedesthatofIL-12 expression.Inaddition,toitsABIlitytocovalentlybindtop35toformIL-12,p40canbindtop19toformIL-23. RecombinantHumanIL-12 p40isa40.0 kDaproteincontaining306 aminoacidresidues.

Source

ChineseHamsterOvaryCells(CHO)

Purity

98%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstitutein1×PBS,pH7.2 toaconcentrationof0.2 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C