Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Stool
Sample Requirements
Serum, Plasma, BALF, Urine, Stool extract: 5 µl/well
CSF: 35 µl/well
For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml).
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1–64 ng/mL
Limit of Detection
0.22 ng/mL
Intra-assay (Within-Run)
n = 8; CV = 5.0%
Inter-assay (Run-to-Run)
n = 6; CV = 4.2%
Spiking Recovery
Serum: 100.7 %
CSF: 99.4 %
Urine: 90.0 %
BALF: 103.7 %
Stool extract: 90.0 %
Dilutation Linearity
Serum: 96.8 %
CSF: 102.5 %
Urine: 97.4 %
BALF: 99.5 %
Stool extract: 110.5 %
Crossreactivity
- bovine Non-detectable
- cat Non-detectable
- dog Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Non-detectable
- chicken Non-detectable
- monkey Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- human Yes
Note
The antibodies used in this ELISA are specific for human S100A8/A9. No crossreactivity has been observed for other human recombinant S100 proteins such as S100A1, A4, A5, A6, A7, A10, A11, A12, A13, A14, A15, A16, S100B and S100G protein at 200 ng/ml. Determination of S100A8/A9 does not interfere with haemoglobin (0.05 mg/ml), bilirubin (170 mmol/l) and triglycerides (5.0 mmol/l).
