Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-Heparin, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Tissue extract
Sample Requirements
5 μl/ well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.125–4 ng/ml
Limit of Detection
0.014 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 5.6%
Inter-assay (Run-to-Run)
n = 6; CV = 6.6%
Crossreactivity
- bovine Non-detectable
- cat Yes (recommended dilution 1:20)
- goat Non-detectable
- horse Non-detectable
- dog Yes (recommended dilution 1:3)
- hamster Yes (recommended dilution 1:6)
- sheep Non-detectable
- chicken Not tested
- human Yes
- pig Yes (recommended dilution 1:3)
- monkey Yes (recommended dilution 1:100)
- mouse Yes (recommended dilution 1:100)
- rabbit Yes (recommended dilution 1:40)
- rat Yes (recommended dilution 1:40)