Type

Sandwich ELISA, HRP-labelled antibody

Applications

Urine, Bronchoalveolar lavage, Milk, Seminal plasma, Plasma (EDTA, citrate, heparin)

Sample Requirements

Plasma: 35 µl/well
Urine: 20 µl/well
Breast milk, BALF, Seminal plasma: 5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box)

Calibration Curve

Calibration Range

1.57–100 ng/ml

Limit of Detection

0.85 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 3.8 %

Inter-assay (Run-to-Run)

n = 6; CV = 12.2 %

Spiking Recovery

Plasma (EDTA): 92.6 %

Dilutation Linearity

Plasma (EDTA): 105.7 % Urine: 100.7 %
Breast milk: 109.4 %
Seminal plasma: 103.5 %
BALF: 115.4 %