Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Urine, Cerebrospinal fluid, Milk, Plasma (EDTA, citrate, heparin)

Sample Requirements

Plasma: 10 µl/well - Serum: 20 µl/well
Urine, CSF, Breast milk: 5 µl/well

Osteopontin is proteolytically cleaved by thrombin during blood clotting, thus, serum osteopontin levels are significantly lower than plasma levels. To prevent OPN cleavage in samples during clotting, PMSF can be added.

Samples should be assayed immediately after collection or should be stored at 20°C or preferably at 70°C. Storage of samples at 4°C should be avoided since it leads to a significant decrease in OPN levels.

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.25 – 16 ng/ml

Limit of Detection

87 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 5.7 %

Inter-assay (Run-to-Run)

n = 6; CV = 3.9 %

Spiking Recovery

EDTA Plasma: 97.2 %
Serum: 90.2 %

Dilutation Linearity

EDTA Plasma: 98.7 %
Serum: 99.42 %