Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-Heparin, Plasma-Citrate, Cell culture medium, Tissue extract

Sample Requirements

20 µl/well

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.2–10 ng/ml

Limit of Detection

Analytical Limit of Detection is calculated from the real Midkine values in wells and is 0.033ng/ml

Intra-assay (Within-Run)

n = 8; CV = 4.5%

Inter-assay (Run-to-Run)

n = 5; CV = 6.3%

Spiking Recovery

98,40%

Dilutation Linearity

103,30%

Crossreactivity