Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin

Sample Requirements

35 µl/well

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2–100 ng/ml

Limit of Detection

Analytical Limit of Detection is calculated from the real Leptin receptor values in wells and is 0.04 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 7.2%

Inter-assay (Run-to-Run)

n = 7; CV = 8.0%

Spiking Recovery

88,60%

Dilutation Linearity

107,90%

Crossreactivity