Type
SandwichELISA,Biotin-labelledantibody
Applications
Serum,Plasma-EDTA,Plasma-Heparin,Plasma-Citrate
SampleRequirements
7 µl/well
Storage/Expiration
Storethekitat2–8°C.Undertheseconditions,thekitisstableuntiltheexpirationdate(seelabelonthe box).
CalibrationCurve
CalibrationRange
100–4000pg/ml
LimitofDetection
AnalyticalLimitofDetectioniscalculatedfromtherealleptinvaluesinwellsandis30 pg/mlformouseleptinand50 pg/mlforrat leptin
Intra-assay(Within-Run)
n=8;CV=2.2%
Inter-assay(Run-to-Run)
n=8;CV=3.4%
SpikingRecovery
94,50%
DilutationLinearity
96,70%
Crossreactivity
- bovineNon-detectable
- catNon-detectable
- dogNon-detectable
- goatNon-detectable
- hamsterNon-detectable
- horseNon-detectable
- monkeyNon-detectable
- pigNon-detectable
- rabbitNon-detectable
- sheepNottested
- chickenNottested
- mouseYes
- ratYes
- humanYes(recommendeddilution1:3)
