Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Milk, Saliva, Stool
Sample Requirements
Serum, Plasma (EDTA), BALF, Breast milk, Saliva: 5 µl/well
CSF, Urine, Stool extract: 20 µl/well
- For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml)
- Special Dilution Buffer is needed for measurement of human lactoferrin in stool extract and is not included.
For protocol for preparation of stool extracts and other details, please contact us at info@biovendor.com
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box)
Calibration Curve
Calibration Range
2.5–80 ng/ml
Limit of Detection
1.1 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 3.3 %
Inter-assay (Run-to-Run)
n = 6; CV = 5.4 %
Spiking Recovery
Serum: 96.6 %
Plasma (EDTA): 103.2 %
BALF: 104.7 %
CSF: 102.3 %
Urine: 104.4 %
Stool extract: 89.7 %
Dilutation Linearity
Serum: 94.6 %
Plasma (EDTA): 97.5 %
BALF: 88.3 %
CSF: 99.5 %
Urine: 98.4 %
Breast milk: 97.1 %
Saliva: 97.1 %
Stool extract: 94.9 %
