Type

SandwichELISA,Biotin-labelledantibody

Applications

Serum,Plasma-EDTA,Plasma-Heparin,Plasma-Citrate,Urine

SampleRequirements

2×5µl/well

Storage/Expiration

Storethekitat2–8°C.Undertheseconditions,thecompletekitisstableuntiltheexpirationdate(seelabelonthe box).

CalibrationCurve

CalibrationRange

10–320µg/l(FLCkappa)
17.5–560µg/l(FLClamBDa)

LimitofDetection

FLCkappaELISALimitofdetection(LOD)(definedasconcentrationofanalytegivingabsorbancehigherthanmeanabsorbanceofblankplusthreestandarddeviationsoftheabsorbanceofblank:Ablank+3×SDblank)iscalculatedfromtherealFLCkappavaluesinwellsandis6 μg/L.

FLClambdaELISALimitofdetection(LOD)(definedasconcentrationofanalytegivingabsorbancehigherthanmeanabsorbanceofblankplusthreestandarddeviationsoftheabsorbanceofblank:Ablank+3×SDblank)iscalculatedfromtherealFLClambdavaluesinwellsandis5 μg/L.

Intra-assay(Within-Run)

n=8;
CV=3.0%(FLCkappa)
CV=4.5%(FLClambda)

Inter-assay(Run-to-Run)

n=6;
CV=7.0%(FLCkappa)
CV=6.4%(FLClambda)

SpikingRecovery

101.4%(FLCkappa)
100.9%(FLClambda)

DilutationLinearity

87.8%(FLCkappa)
85.8%(FLClambda)

Crossreactivity