Type
SandwichELISA,AChE-labelledantibody
Applications
Plasma-EDTA
SampleRequirements
10 µl/well
Storage/Expiration
Storethekitat –20°C.Undertheseconditions,thekitisstableuntilexpirationdate(seelabelonthe box).
CalibrationRange
1.96–250pg/ml
LimitofDetection
TheLimitofDetermination,calculatedastheconcentrationofunacylatedghrelincorrespondingtotheNSBaverage(n=8)plusthreestandarddeviationsis0.6 pg/mland0.8 pg/mlforlongandshortfirstincubationperiod,respectively.
Intra-assay(Within-Run)
CV=8.2%(20hat+4°C)
CV=9.2%(3hatRT)
Inter-assay(Run-to-Run)
CV=8.6%(20hat+4°C)
CV=9.5%(3hatRT)
SpikingRecovery
101.5%(20hat+4°C)
99.8%(3hatRT)
DilutationLinearity
98,90%
