Type
SandwichELISA,AChE-labelledantibody
Applications
Plasma-EDTA+PHMB
SampleRequirements
20 µl/well
Storage/Expiration
Storethekitat –20°C.Undertheseconditions,thekitisstableuntilexpirationdate(seelabelonthe box).
CalibrationCurve
CalibrationRange
1.96–250pg/ml
LimitofDetection
TheLimitofDetermination,calculatedastheconcentrationofacylatedghrelincorrespondingtotheNSBaverage(n=8)plusthreestandarddeviationsis0.3 pg/mland0.8 pg/mlforlongandshortfirstincubationperiod,respectivelyforlongandshortimmunologicalreaction.
Intra-assay(Within-Run)
CV=8.0%(20hat+4°C)
CV=7.0%(3hatRT)
Inter-assay(Run-to-Run)
CV=8.4%(20hat+4°C)
CV=8.2%(3hatRT)
SpikingRecovery
101.6%(20hat+4°C)
111.0%(3hatRT)
DilutationLinearity
89,20%
